CCR5/Gα15/CHO
CBP71057
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產(chǎn)品描述
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| I. Background | |
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CCR5（趨化因子受體 5 或趨化因子 C－C 亞族受體 5），也稱為 CD195，是一種 G蛋白 偶聯(lián)受體，屬于整合型膜蛋白的 β 趨化因子受體家族成員。CCR5 主要與 Gα i/o 和 Gα q/11 偶聯(lián)，在樹突狀細胞、T 細胞、巨噬細胞、嗜酸性粒細胞、小膠質(zhì)細胞和髓系抑制細胞上 表達，并由多種配體（CCL3、CCL3L1、CCL4、CCL5、CCL8、CCL11、CCL13 和 CCL16） 激活，以促進趨化性和免疫細胞活化。
巨噬細胞和樹突狀細胞分泌 CCR5 依賴性促炎細胞因子可激活適應(yīng)性免疫反應(yīng)?；罨?的 CD4+ T 細胞分泌 CCR5 依賴性 IL-2 可誘導(dǎo)效應(yīng) T 細胞、記憶 T 細胞和調(diào)節(jié)性 T 細胞的 增殖和活化。 CCR5 是加速募集效應(yīng)和記憶 CD8+ T 細胞至感染部位所必需的。CCR5 是 HIV 進入免疫細胞的輔助受體。CCR5 可與多種配體任意結(jié)合，從而啟動包括引導(dǎo)遷移在 內(nèi)的細胞信號傳導(dǎo)。 |
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| II. Introduction | |
| Host Cell: |
CHO |
| Stability: | 20 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
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Gene Synonyms: |
C-C chemokine receptor type 5, CCR5 |
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Expressed Gene: |
GenBank accession number NM_000579; no expressed tags |
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Host Cell: |
CHO-K1 |
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Stability: |
32 passages |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: | F-12+10%FBS+3μg/ml puromycin+100μg/ml Hygromycin B |
| Mycoplasma Status: | Negative |
| Storage: | Liquid nitrogen immediately upon delivery |
| Application(s): |
Functional assay for CCR5 |
| Transducer: | Gi |
| Ⅲ. Description of Host Cell Line | |
| Organism: | Hamster |
| Tissue: | Ovary |
| Morphology: | Epithelial |
| Growth Properties: | Adherent |
| Ⅳ. Representative Data | |
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Concentration-dependent stimulation of intracellular calcium mobilization by MIP-1α in CCR5/Gα16/CHO and Gα15/CHO cells Figure 1. MIP-1α-induced concentration-dependent stimulation of intracellular calcium mobilization in CCR5/Gα16/CHO and Gα15/CHO cells. The cells were loaded with Calcium-4 prior to stimulation with an CCR5 receptor agonist, MIP-1α. The intracellular calcium change was measured by FlexStation. |
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