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MCF7-Luc

CBP30105L

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I. Introduction
Host Cell: MCF7 ER positive
Expressed gene: Luciferase
Stability: 32 passages(in-house test, that not means the cell line will be instable beyond the passages we tested.)
Freeze Medium: 90% FBS+10% DMSO
Culture Medium: MEM + 10% FBS+ 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP)+ 10 µg/ml human insulin+ 1μg/ml puromycin
Mycoplasma Status: Negative
Storage: Liquid nitrogen
 
II. Description of Host Cell Line
Organism: Homo sapiens,human
Tissue: Mammary gland breast;derived from metastaticsite:pleural effusion
Disease: Adenocarcinoma
Morphology: Epithelial
Growth Properties: Adherent
 
III. Representative Data
CBP30105L 信號(hào)

Figure. Detect Luciferase assay by Promega Bright-Glo Luciferase Assay System..
The number of Luminescence signal is 497±5(Mean±SD) on MCF7 cells while 979200±36119(Mean±SD) on MCF7-Luc cells.

 
IV. Stablity Test
CBP30105L 穩(wěn)定性

Figure. Stablity test results. MCF7-Luc cells showed at least 32 passage stability with similar luminescence unit.


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